《植物生理学报》 2009, 45(1): 33-36

低温下转昆虫抗冻蛋白基因烟草的亚显微结构变化

王艳, 马纪*, 邱立明, 王晶, 代春英, 张富春

新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 乌鲁木齐830046

通信作者：马纪；E-mail: majiuci@xju.edu.cn；Tel: 0991-8583517

摘　要：

分析在 -1 ℃温度中处理 1、2 和 3 d 的转昆虫抗冻蛋白基因 MpAFP149 烟草和野生型烟草亚细胞显微结构变化的结 果表明, 转基因烟草和野生型烟草的超微结构有差异, 尤其是细胞膜、叶绿体和线粒体的膜。转基因烟草细胞器的膜结构 伤害程度明显低于野生型烟草, 这提示低温条件下转基因烟草中的抗冻蛋白可能有保护细胞膜和细胞器膜结构的作用, 由 此降低了低温的伤害。

关键词：转抗冻蛋白基因烟草; 低温胁迫; 超微结构

收稿：2008-09-16   修定：2008-11-27

资助：国家自然科学基金(30760056)和教育部春晖计划项目(Z2004-2-65040)。

Changes of Ultrastructure of Transgenic Tobacco (Nicotiana tabacum L.) with Insect Antifreeze Protein Gene under –1 ℃ Condition

WANG Yan, MA Ji*, QIU Li-Ming, WANG Jing, DAI Chun-Ying, ZHANG Fu-Chun

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang

Corresponding author: MA Ji; E-mail: majiuci@xju.edu.cn; Tel: 0991-8583517

Abstract:

The ultrastructures in transgenic tobacco (Nicotiana tabacum) seedlings containing insect antifreeze gene were analyzed under –1 ℃ for 0, 1, 2 and 3 d. The results showed that the ultrastructures in transgenic tobacco seedlings were different from those in wild tobacco plants, especially the membranes of cellular organelles including cell membranes, chloroplast membranes and mitochondria membranes. The observation of transmission electron microscope (TEM) showed that the damage degree of transgenic tobacco was lower than that of wild tobacco, which suggested that the insect antifreeze protein could protect cell membrane and the membranes of cellular organelles under low temperature, and reduce the damage degree of low temperature.

Key words: transgenic tobacco carrying insect antifreeze protein gene; cold treatment; ultrastructure